Ano1 Knockout cell line (Raw 264.7)

Ano1 Knockout cell line (Raw 264.7) Order

Catalog#

YKO-MT0045

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Ano1 Knockout cell line (Raw 264.7)

Species

Mouse

Gene Name

Ano1

Gene ID

101772

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 2 (based on transcript Ano1-211)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Ano1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Raw 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Ano1 gene knockout Ano1 Knockout cell line (Raw 264.7) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Ano1 Knockout cell line (Raw 264.7)

Catalog#

YKO-MT0045

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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Ano1 Knockout cell line (Raw 264.7)

Ubigene